Extraction of protein and amino acids from deoiled rice bran by subcritical water hydrolysis
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Issara Sereewatthanawut, Surawit Prapintip, Katemanee Watchiraruji, Motonobu Goto, Mitsuru Sasaki and
Artiwan Shotipruk
Department of Chemical Engineering, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok
10330, Thailand
Department of Applied Chemistry and Biochemistry, Kumamoto University, Kumamoto 860-8555, Japan
Received 2 November 2006;revised 28 December 2006;accepted 28
December 2006. Available online 22 February 2007.

Abstract

This study investigated the production of value-added protein and amino acids from deoiled rice bran by
hydrolysis in subcritical water (SW) in the temperature range between 100 and 220 for 0–30 min. The

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results suggested that SW could effectively be used to hydrolyze deoiled rice bran to produce useful protein
and amino acids. The amount of protein and amino acids produced are higher than those obtained by
conventional alkali hydrolysis. The yields generally increased with increased temperature and hydrolysis
time. However, thermal degradation of the product was observed when hydrolysis was carried out at higher
temperature for extended period of time. The highest yield of protein and amino acids were 219 ± 26 and
8.0 ± 1.6 mg/g of dry bran, and were obtained at 200 at hydrolysis time of 30 min. Moreover, the product

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obtained at 200

after 30 min of hydrolysis exhibited high antioxidant activity and was shown to be

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suitable for use as culture medium for yeast growth.

Key words: Hydrolysis; Rice bran; Proteins; Amino acids; Hydrothermal

摘

要

本实验研究的是增值蛋白质和氨基酸的生产，生产方法为脱油米糠在亚临界水

中的水解，水解温度 100 至 220℃，时间为 0-30 分钟。结果表明，亚临界水可有效
地用于水解米糠脱油生产有用的蛋白质和氨基酸。蛋白质和氨基酸生产的数量比传统
碱性水解得到的高。产量的提升与温度，水解时间的增加有关。不过，在长时间的高
温下，水解度较高时进行时，即在水解温度 200℃，水解时间 30 分钟，对该产品的
热降解进行观察时，产量最高的蛋白质和氨基酸含量为 219 ± 26 和 8.0 ± 1.6 毫克
/克干麸皮。此外，该产品在 200℃，30 分钟以后的水解具有很高的抗氧化活性，更
适合作为传统的酵母培养基使用。

关键词：水解；米糠；蛋白质；氨基酸；水浴

Preparation and Functional Properties of Rice Bran Protein Isolate

M. Wang, N. S. Hettiarachchy, M. Qi, W. Burks, and T. Siebenmorgen